Development and characterisation of a low-concentration sodium dodecyl sulphate decellularised porcine dermis

Development and characterisation of a low-concentration sodium dodecyl sulphate decellularised porcine dermis

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The aim of this study was to adapt a proprietary decellularisation process for human dermis for use with porcine skin.Porcine skin was subject to: sodium chloride (1 M) tropical hideaway beer to detach the epidermis, trypsin paste to remove hair follicles, peracetic acid (0.1% v/v) disinfection, washed in hypotonic buffer and 0.

1% (w/v) sodium dodecyl sulphate in the presence of proteinase inhibitors followed by nuclease treatment.Cellular porcine skin, decellularised porcine and human dermis were compared using histology, immunohistochemistry, GSL-1 lectin (alpha-gal epitope) staining, biochemical assays, uniaxial tensile and in vitro cytotoxicity tests.There was no microscopic evidence of cells in decellularised porcine dermis.

DNA content was vive night splint reduced by 98.2% compared to cellular porcine skin.There were no significant differences in the biomechanical parameters studied or evidence of cytotoxicity.

The decellularised porcine dermis retained residual alpha-gal epitope.Basement membrane collagen IV immunostaining was lost following decellularisation; however, laminin staining was retained.